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packaging plasmids pspax2  (Addgene inc)


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    Structured Review

    Addgene inc packaging plasmids pspax2
    Packaging Plasmids Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspax2+packaging+plasmids/psPAX2+(Plasmid+%2312260)/pmc13042270-94-6-12
    Average 98 stars, based on 15460 article reviews
    packaging plasmids pspax2 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Produced:

    Article Title: HRP2 regulating MICU1-mediated Ca 2+ overload to dictate chemoresistance of multiple myeloma.
    Article Snippet: Despite the efficacy of bortezomib (BTZ)-based chemotherapy in treating multiple myeloma (MM) patients, chemoresistance occurs frequently over time, particularly in individuals exhibiting an initial positive response to BTZ therapy.. In this study, we established BTZ-resistant MM cells and identified that suppressed expression of the hepatoma-derived growth factor (HDGF)-related protein-2 (HRP2) was a key determinant of chemoresistance in MM cells.. Manipulating HRP2 expression remodeled the chemosensitivity of MM cells in vitro and in vivo.

    Article Title: HRP2 regulating MICU1-mediated Ca 2+ overload to dictate chemoresistance of multiple myeloma
    Article Snippet: Transient transfections were performed on HEK293T cells using polyethyleneimine (PEI) (Polysciences) in the OPTI-MEM medium (Life Technologies) with a DNA: PEI ratio ranging from 1:4 to 1:6. .. Viral particles were produced by transfecting HEK293T cells in a 10 cm dish with 4 μg pMD2.G and 6 μg psPAX2 packaging plasmids (Addgene), together with 8 μg lentiviral expressing vectors encoding target genes, including pITA-HRP2, pITA-MICU1, pLKO.1 vector encoding shRNAs targeting HRP2 and MICU1 genes. .. The supernatant carrying the viral particles was harvested at 36 h and 60 h after transfection and concentrated to 100 × volume by Poly (ethylene glycol) 8000 (Sigma-Aldrich).

    Expressing:

    Article Title: HRP2 regulating MICU1-mediated Ca 2+ overload to dictate chemoresistance of multiple myeloma.
    Article Snippet: Despite the efficacy of bortezomib (BTZ)-based chemotherapy in treating multiple myeloma (MM) patients, chemoresistance occurs frequently over time, particularly in individuals exhibiting an initial positive response to BTZ therapy.. In this study, we established BTZ-resistant MM cells and identified that suppressed expression of the hepatoma-derived growth factor (HDGF)-related protein-2 (HRP2) was a key determinant of chemoresistance in MM cells.. Manipulating HRP2 expression remodeled the chemosensitivity of MM cells in vitro and in vivo.

    Article Title: HRP2 regulating MICU1-mediated Ca 2+ overload to dictate chemoresistance of multiple myeloma
    Article Snippet: Transient transfections were performed on HEK293T cells using polyethyleneimine (PEI) (Polysciences) in the OPTI-MEM medium (Life Technologies) with a DNA: PEI ratio ranging from 1:4 to 1:6. .. Viral particles were produced by transfecting HEK293T cells in a 10 cm dish with 4 μg pMD2.G and 6 μg psPAX2 packaging plasmids (Addgene), together with 8 μg lentiviral expressing vectors encoding target genes, including pITA-HRP2, pITA-MICU1, pLKO.1 vector encoding shRNAs targeting HRP2 and MICU1 genes. .. The supernatant carrying the viral particles was harvested at 36 h and 60 h after transfection and concentrated to 100 × volume by Poly (ethylene glycol) 8000 (Sigma-Aldrich).

    Article Title: Delactylation of the tumor suppressor ARHGDIB drives metastasis and chemoresistance in bladder cancer.
    Article Snippet: Cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific). .. The viral supernatant was harvested 48 h following co-transfection with lentiviral expression vectors (Ubi-MCS-3FLAG-CBhgcGFP-IRES-puromycin) and pMD2.G and psPAX2 packaging plasmids (Addgene, Watertown, MA, USA). ..

    Plasmid Preparation:

    Article Title: HRP2 regulating MICU1-mediated Ca 2+ overload to dictate chemoresistance of multiple myeloma.
    Article Snippet: Despite the efficacy of bortezomib (BTZ)-based chemotherapy in treating multiple myeloma (MM) patients, chemoresistance occurs frequently over time, particularly in individuals exhibiting an initial positive response to BTZ therapy.. In this study, we established BTZ-resistant MM cells and identified that suppressed expression of the hepatoma-derived growth factor (HDGF)-related protein-2 (HRP2) was a key determinant of chemoresistance in MM cells.. Manipulating HRP2 expression remodeled the chemosensitivity of MM cells in vitro and in vivo.

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor.
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; SigmaAldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [19]. ..

    Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS
    Article Snippet: LentiX-HEK293T cells (Takara Bio) were cultured in DMEM with 10% FBS and 1mM sodium pyruvate. .. Lentiviral transfer plasmid was transiently transfected using Lipofectamine 2000 transfection reagent (ThermoFisher, Cat. #11668500) alongside pMD2.G and psPAX2 packaging plasmids (Trono Lab, Addgene ##12259; ##12260) to create VSV-G pseudotyped lentiviral particles. .. Lentivirus was harvested 48-72h post-transfection and concentrated via ultracentrifugation (25,000g, 2h, 4°C), then resuspended in StemSpan SFEM II (Stemcell Technologies).

    Article Title: HRP2 regulating MICU1-mediated Ca 2+ overload to dictate chemoresistance of multiple myeloma
    Article Snippet: Transient transfections were performed on HEK293T cells using polyethyleneimine (PEI) (Polysciences) in the OPTI-MEM medium (Life Technologies) with a DNA: PEI ratio ranging from 1:4 to 1:6. .. Viral particles were produced by transfecting HEK293T cells in a 10 cm dish with 4 μg pMD2.G and 6 μg psPAX2 packaging plasmids (Addgene), together with 8 μg lentiviral expressing vectors encoding target genes, including pITA-HRP2, pITA-MICU1, pLKO.1 vector encoding shRNAs targeting HRP2 and MICU1 genes. .. The supernatant carrying the viral particles was harvested at 36 h and 60 h after transfection and concentrated to 100 × volume by Poly (ethylene glycol) 8000 (Sigma-Aldrich).

    Article Title: PLK1 blockade enhances the anti-tumor effect of MAPK inhibition in pancreatic ductal adenocarcinoma.
    Article Snippet: .. A plasmid pool of three to five lentiviral shRNA plasmids targeting the PLK1 gene (Cat. # sc-36277-SH, Santa Cruz Biotechnology, Dallas, USA), the pCMV-VSV-G envelope plasmids (Cat. #8454, Addgene, MA, USA) and psPAX2 packaging plasmids (Cat. #12260, Addgene, Watertown, USA) were used for PLK1 knockdown in PaTu 8988t, PaTu 8988s, PANC-1, CFPAC, CDX85 and CDX89 cell lines. .. A plasmid pool of three to five lentiviral shRNA plasmids targeting the PLK1 gene (Cat. # sc-36277-SH, Santa Cruz Biotechnology, Dallas, USA), the pCMV-VSV-G envelope plasmids (Cat. #8454, Addgene, MA, USA) and psPAX2 packaging plasmids (Cat. #12260, Addgene, Watertown, USA) were used for PLK1 knockdown in PaTu 8988t, PaTu 8988s, PANC-1, CFPAC, CDX85 and CDX89 cell lines.

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; Sigma-Aldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [ ]. ..

    Control:

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor.
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; SigmaAldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [19]. ..

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; Sigma-Aldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [ ]. ..

    shRNA:

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor.
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; SigmaAldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [19]. ..

    Article Title: PLK1 blockade enhances the anti-tumor effect of MAPK inhibition in pancreatic ductal adenocarcinoma.
    Article Snippet: .. A plasmid pool of three to five lentiviral shRNA plasmids targeting the PLK1 gene (Cat. # sc-36277-SH, Santa Cruz Biotechnology, Dallas, USA), the pCMV-VSV-G envelope plasmids (Cat. #8454, Addgene, MA, USA) and psPAX2 packaging plasmids (Cat. #12260, Addgene, Watertown, USA) were used for PLK1 knockdown in PaTu 8988t, PaTu 8988s, PANC-1, CFPAC, CDX85 and CDX89 cell lines. .. A plasmid pool of three to five lentiviral shRNA plasmids targeting the PLK1 gene (Cat. # sc-36277-SH, Santa Cruz Biotechnology, Dallas, USA), the pCMV-VSV-G envelope plasmids (Cat. #8454, Addgene, MA, USA) and psPAX2 packaging plasmids (Cat. #12260, Addgene, Watertown, USA) were used for PLK1 knockdown in PaTu 8988t, PaTu 8988s, PANC-1, CFPAC, CDX85 and CDX89 cell lines.

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; Sigma-Aldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [ ]. ..

    Transfection:

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor.
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; SigmaAldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [19]. ..

    Article Title: Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS
    Article Snippet: LentiX-HEK293T cells (Takara Bio) were cultured in DMEM with 10% FBS and 1mM sodium pyruvate. .. Lentiviral transfer plasmid was transiently transfected using Lipofectamine 2000 transfection reagent (ThermoFisher, Cat. #11668500) alongside pMD2.G and psPAX2 packaging plasmids (Trono Lab, Addgene ##12259; ##12260) to create VSV-G pseudotyped lentiviral particles. .. Lentivirus was harvested 48-72h post-transfection and concentrated via ultracentrifugation (25,000g, 2h, 4°C), then resuspended in StemSpan SFEM II (Stemcell Technologies).

    Article Title: The Selective 3-MST Inhibitor I3MT-3 Works as a Potent Caspase-1 Inhibitor
    Article Snippet: .. The 3-MST or control shRNA (pLKO.1) lentivirus vector (MISSON shRNA; Sigma-Aldrich) was transfected with the VSV-G envelope and psPAX2 packaging plasmids (Addgene) into HEK293A cells using PEI-MAX as previously described [ ]. ..

    Knockdown:

    Article Title: PLK1 blockade enhances the anti-tumor effect of MAPK inhibition in pancreatic ductal adenocarcinoma.
    Article Snippet: .. A plasmid pool of three to five lentiviral shRNA plasmids targeting the PLK1 gene (Cat. # sc-36277-SH, Santa Cruz Biotechnology, Dallas, USA), the pCMV-VSV-G envelope plasmids (Cat. #8454, Addgene, MA, USA) and psPAX2 packaging plasmids (Cat. #12260, Addgene, Watertown, USA) were used for PLK1 knockdown in PaTu 8988t, PaTu 8988s, PANC-1, CFPAC, CDX85 and CDX89 cell lines. .. A plasmid pool of three to five lentiviral shRNA plasmids targeting the PLK1 gene (Cat. # sc-36277-SH, Santa Cruz Biotechnology, Dallas, USA), the pCMV-VSV-G envelope plasmids (Cat. #8454, Addgene, MA, USA) and psPAX2 packaging plasmids (Cat. #12260, Addgene, Watertown, USA) were used for PLK1 knockdown in PaTu 8988t, PaTu 8988s, PANC-1, CFPAC, CDX85 and CDX89 cell lines.



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